Maize single cell sequencing
Web1 day ago · A high-throughput single-cell single-mitochondrial genome sequencing technology known as iMiGseq has provided new insights into mutations of mitochondrial … WebOct 1, 2024 · We captured 4577, 5054, and 7241 cells in the AC, MMC1, and MMC2-stage ovule-primordium samples, respectively, for single-cell RNA-seq library construction and sequencing and obtained 192,137 ...
Maize single cell sequencing
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WebFeb 21, 2024 · As an emerging sequencing technology, single-cell RNA sequencing (scRNA-Seq) has become a powerful tool for describing cell subpopulation classification and cell heterogeneity by achieving high-throughput and multidimensional analysis of individual cells and circumventing the shortcomings of traditional sequencing for detecting the … WebFeb 22, 2024 · Isolation of maize ear protoplasts to construct a single-cell transcriptomic atlas (A) Experimental design, the first panel shows a scanning electron microscope …
WebJan 7, 2024 · Here, we used single-cell RNA sequencing to investigate BS differentiation in C 4 maize ( Zea mays L.) plants. Abaxial BS ( ab BS) cells of rank-2 intermediate veins specifically expressed three SWEET sucrose uniporters (SWEET13a, b, and c) and UmamiT amino acid efflux transporters. WebSingle-cell RNA sequencing of developing maize ears facilitates functional analysis and trait candidate gene discovery Highlights • scRNA-seq of developing maize ears reveals …
Web2 days ago · The global Single Cell Sequencing market size is projected to grow from USD million in 2024 to USD million in 2029; it is expected to grow at a CAGR of Percent from 2024 to 2029. WebHere, we investigated the transcriptional profiles of 12,525 single cells from developing maize ears. The resulting developmental atlas provides a single-cell RNA sequencing (scRNA-seq) map of an inflorescence. We validated our results by mRNA in situ hybridization and by fluorescence-activated cell sorting (FACS) RNA-seq, and we show …
WebSingle-cell sequencing approaches reveal the intracellular dynamics of individual cells and answer biological questions with high-dimensional catalogs of millions of cells, including … if i were an animal i would be a birdWebMar 2, 2024 · Maize single cell sequencing dataset 1: Sample type: SRA : Source name: maize (B73) leaf 2: Organism: Zea mays: Characteristics: genotype: B73 inbred line tissue: V2 seedling leaf 2, distal 6cm: Treatment protocol: Protoplasts for scRNA-seq were generated from the last 6 cm of the distal portion of V2 leaf 2. For each pool of … if i were an animal i would be a lion essayWebThe allele-specific polymerase chain reaction (ASPCR) has been used to determine the genotype of maize lines at two loci, wx and NPI288. The ASPCR method uses allele … if i were an animal i would be a lionWeb2.3 Single-cell library preparation and sequencing Six thousand cells per sample were targeted for capture. The cell suspension of each sample was run in the Chromium Controller with appropriate reagents to generate single-cell gel bead-in-emulsions (GEMs) for sample and cell barcoding. is spring potential energy always positiveWebMay 7, 2024 · The project, led by Alexandre Marand, applied single-cell sequencing technology to break up tissues and organs from maize, isolating thousands of individual cells to determine the regulatory DNA—the parts of DNA that control the genes that are important in different types of cells. if i were an animal i would be a dolphinWebApr 12, 2024 · Single-cell sequencing would allow you to examine and identify the components of each fruit (strawberries, oranges, kiwis, grapes). This gives a very clear picture of the genomes from each cell type and allows you to identify a potential gene, or target, that can only be found in the strawberries so that whatever therapy or drug you … if i were an animal i would be a tigerWebFeb 15, 2024 · A cell atlas of maize peels and leaf bases created by single-nucleus RNA sequencing provides insights into the molecular basis of stomatal movement and developm. ... We have developed a planta platform of single-nucleus RNA-sequencing by combined fluorescence-activated nuclei flow sorting, and used it to identify cell types in … is spring rolls chinese