Hepg2 rnaimax
Web11 apr. 2024 · Polyethylenimine (Polysciences) and Lipofectamine RNAiMAX (Invitrogen) were used to transfect plasmids and miRNAs, respectively, according to the manufacturer’s instructions. β-Actin antibodies (sc-47778) and anti-mouse horseradish peroxidase (HRP)-conjugated IgG (sc-2748) were purchased from Santa Cruz Biotechnology. WebThe siRNAs were transfected into the HepG2 and Huh7 cells using Lipofectamine RNAiMAX reagent (Invitrogen). The cells were re-transfected after the first transfection …
Hepg2 rnaimax
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WebLipofectamine® RNAiMAX Transfection Reagent is a proprietary formulation for transfecting small RNAs \(e.g., siRNA, Silencer® Select siRNA, Stealth® RNAi, mirVana miRNA … WebLipofectamine 2000 - Thermo Fisher Scientific - US
WebPROTOCOL The following is a general protocol for use of DharmaFECT™ transfection reagents to deliver siRNA into cultured mammalian cells. The examples WebThree cell densities of HepG2 cells were transfected with GAPD siRNA (100 nM) using a range of volumes (0.05 to 1.6 μL/well) of Lipofectamine 2000 and DharmaFECT 4 …
Webfectamine RNAiMAX with very low cell toxicity. Methods Systematic design of experiments used for screening transfection reagents The screening of transfection reagents was con … WebGeneral Transfection Protocols. Cell-Specific Transfection Protocols. Transfection Reagents - Product Protocols. NOTE: Invitrogen Lipofectamine Transfection Reagent Protocols …
Web30 mrt. 2024 · Pectolinarigenin (PG) and its glycoside pectolinarin (PN) were reported to have various health beneficial functions such as anti-inflammatory and anti-carcinogenic …
Web15 feb. 2024 · We overexpressed hsa-miR-193b-3p mimic in HepG2 cells for 72 h, under normoglycemia (5 mM glucose) and hyperglycemia (20 mM glucose); visualized lipid droplet content using Oil Red O staining and brightfield microscopy ( Figure 2 ); and performed quantitative image analysis. sharper image light up electronic word clockWeb15 feb. 2024 · Overexpression of miR-193b-3p in HepG2 cells. microRNA miR-193b-3p overexpression in HepG2 cells was performed over 72 h by reverse transfection on the … sharper image literati manualhttp://www.accesolab.com/wpress/wp-content/uploads/2014/07/manual-lipofectamina.pdf sharper image laser hair removalWebHepG2 cells, GAPDH silencing obtained by using GenMute was significant with respect to its relative control only after 24 h of treatment with the transfection agent ( Figure 5 A). … sharper image levitating light bulbWeb11 mei 2024 · The HepG2 and sorafenib resistant HepG2-R cells were obtained from Yang Liu lab (China Medical University, Liaoning, China). HepG2-R cells were established with … pork loin sell by dateWeb13778-075, 13778-150). Lipofectamine™ RNAiMAX has a broad range of activity, enabling achievement of maximal knockdown levels with a minimum of optimization required. … pork loin sirloin half recipeWebHepG2 cells as an in vitro transient expression assay system is that efficient gene silencing is difficult to achieve with many commonly used chemical methods. Among the methods … pork loin sauces for pork loin