WebApr 2, 2024 · Cell lysate (500 μg) was labeled according to the Click-iT O-GlcNAc Enzymatic Labeling System protocol (Invitrogen), and conjugated with an alkyne–biotin compound as per the Click-iT Protein Analysis … WebSep 14, 2008 · Labelled proteins were biotinylated (Click-iT protein analysis detection kit) and then purified by using streptavidin– agarose beads (Invitrogen); final products were subjected to immunoblot analyses. Quantitative real-time PCR. Total RNA samples were prepared with TRIzol (Invitrogen) and quantified by spectrophotometry. RNA (5 μg) was ...
Click-iT™ Protein Reaction Buffer Kit - Thermo Fisher …
WebThe ProteoSpin™ Urine Protein Concentration Midi Kit provides a fast and simple procedure for concentrating dilute solutions of urine proteins from 1 to 5 mL inputs of urine. Each mini spin column is able to concentrate and … WebDetection utilizes the chemoselective ligation or 'click' reaction between an azide and an alkyne where the azido modified protein is detected with one of the Click-iT Protein Analysis Detection Kits containing either TAMRA, Dapoxyl™, or biotin alkyne. finegroomer.com
O-GlcNAcylation regulates the methionine cycle to …
WebJan 7, 2024 · The experiment was performed based on the Click-iT O-GlcNAc Enzymatic Labeling System protocol, and the Click-iT Protein Analysis Detection Kit protocol (Life Technologies). Control experiments were carried out in parallel in the absence of the labeling enzyme GalT or UDP-GalNAz. The level of O-GlcNAcylation was determined by … WebCopper-less Click Chemistry Reagents - Molecular Probes - Invitrogen EN English Deutsch Français Español Português Italiano Român Nederlands Latina Dansk Svenska Norsk Magyar Bahasa Indonesia Türkçe Suomi Latvian … WebMay 6, 2010 · The chemoselective ligation between the azide and alkyne was performed using the Click-iT Protein Analysis Detection Kit (no. MP33370; Invitrogen), and the binding of fluorescent dye was analyzed by confocal microscopy. Statistical analyses fine grits